Proficiency Courses Biochem/Biotech/Immunology – JIVAS Bengaluru
Proficiency Courses Biochem/Biotech/Immunology – JIVAS Bengaluru
Jain institute of vocational and advance studies
# 127/3 , Bull Temple Road , Chamrajpet
Bengaluru 560 019
Proficiency Courses:
These courses are basically designed to make the students proficient in the different techniques used in Biotechnology. The students will be exposed to advanced areas of Bioscience which will give them a cutting edge in enhancing the employment opportunity but also the academic advancement. The programe lays more emphasis on hands on training. It gives a thorough practical exposure in handling the most principally used techniques in different avenues of Biotechnology.
The modules are:
Agricultural Microbiology (20 days)
Isolation of :
Cellulose degrading Microbes
Pectin degrading Microbes
Starch degrading Microbes
Lignin degrading Microbes
• Isolation of Rhizobium from Root Nodules of legumes
• Isolation of non-symbiotic Azotobacter from Soil
• Isolation of Associative Nitrogen fixer – Azospirillum
• Demonstration of Cyanobacterial Symbiosis in Azolla
• Demonstration of Nitrate reduction by Bacteria
• Assay of Biofertilizers
• Mushroom cultivation
• Estimation of Protein and carbohydrate content of mushroom
• Production of Synthetic Seeds
• Vermi -composting
Food Microbiology (20 days)
• Isolation of Microbes from fermented foods
• Study of Aflatoxin producing fungi- Aspergillus flavus
• Isolation of microbes from infected fruits and vegetables
• Isolation of microbes from dairy products
• Production and Estimation of Citric acid from Aspergillus niger
• Quality analysis of water by MPN test
• Production of Bio-yoghurt
• Quality analysis of canned food
• Quality analysis of milk
• Enumeration of microbes in food by Haemocytometer
• Production and estimation of Alcohol from Yeast
• Antimicrobial compounds in food
Immunology (20 days)
• Blood collection, separation of serum and plasma
• Separation of serum proteins on native PAGE
• Blood Smear preparation and Visualization of Blood Cells
• Blood Grouping
• Counting of RBCs, WBCs, and Platelets
• Estimation of Haemoglobin using haemocytometer
• Erythrocyte sedimentation rate count
• Counter Current Immunoelectrophoresis
• PCV
• Widal, RPR, RA, and Latex agglutination test
• Measurement of Serum Albumin
• Separation of Serum Amino acids by Paper Chromatography
• RID, RIEP, and ODD
DNA Science (20 days)
• Genomic DNA isolation from Bacteria
• Genomic DNA isolation from Plants
• Genomic DNA isolation from Blood
• Plasmid DNA isolation
• RNA isolation from yeast
• Agarose Gel electrophoresis
• Gel Elution
• Restriction Digestion
• Ligation
• Polymerase Chain Reaction
• Conjugation
• Phage Titration
• Transformation
Plant Biotechnology (20 days)
• Stock solution and Chemical preparation
• Media preparation
• Production of Synthetic seeds
• Agrobacterium mediated transformation
• Protoplast isolation
• Genomic DNA isolation from plant sample
In vitro culturing technique
Primary Culture:- Seed, Embryo, Nodal, Internodal, Leaf , Storage organ, Nucellus, Anther, Shoot tip, Root tip, Suspension culture
Sub culture:- Callus, Shoot induction, Root induction, Embryo induction
Clinical Biochemistry (20 days)
• Qualitative analysis of Urine
• Estimation of blood Glucose
• Estimation of Serum Proteiness
• Estimation of serum Albumin – Globulin ratio
• Estimation of Serum SGOT, SGPT, LDH
• Estimation of DNA
• Estimation of RNA
• Estimation of Uric acid
• Estimation of Cholesterol.
• Lipid Profile test
• Estimation of free fatty acids
• Thin Layer Chromatography
• Estimation of Serum Amino cids
• Assay of Enzymes for the effect of pH , Temperature, Enzyme Concentration, Substrate Concentration,
Activators, Inhibitors
Industrial Biotechnology (20 days)
Isolation, Screening and Mass production of industrially important micro organisms
( a ) Enzyme producing microorganism
( b ) Antibiotic producing microorganism
( c ) Microorganisms used as biofertilizers
• Immobilization of Enzymes/cells
• Wine Production and Estimation of alcohol content in the produced wine
• Study of Growth curve of microorganisms
• Study of fermenter operations
• Precipitation
• Dialysis
• Chromatography
Chromatographic Techniques ( 10 days)
• Paper Chromatography
• Thin Layer Chromatography
• Ion exchange Chromatography
• Gel Filtration Chromatography
• Affinity Chromatography
• HPLC Analysis of pharmaceutical formulations
Source:
www.jivas.ac.in
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