Biotech/Bioinformatics Project Fellowship JIVAS Bengaluru
JIVAS – Enzymology/Plant Biotechnology Projects Bengaluru
Jain institute of vocational and advance studies
# 127/3 , Bull Temple Road , Chamrajpet
Bengaluru 560 019
Projects
- HPLC
- Enzymology
- Fermentation Technology
- Plant Biotechnology
- Molecular Biology
- Bioinformatics
HPLC
- The need for high-resolution separation methods for analytical and preparative purposes is growing by the HPLC which is a state-of the-art technique that is ideal for such requirements due to its precision, sensitivity and reproducibility.
- HPLC training involving qualitative and quantitative analysis of samples like antibiotics, pesticides etc.at different levels.
Courses Offered:
Advanced Module:
- History and Principle
- Instrumentation and working
- Software usage
- Solvent preparation
- Sample injection
- Result interpretation
- Estimation of agrochemicals/pharmaceutical compounds using a pre-set method
Method Development
- History and Principle
- Instrumentation and working
- Software usage
- Result interpretation
- Spectrum scanning
- Solvent selection
- Solvent preparation
- Sample injections
- Optimization of separation by varying different conditions
- Selection of Internal Standard
- Estimation of Active Ingredient in samples using optimized conditions
Enzymology
- Life is controlled by a set of biochemical algorithms and all life reactions abide by this logic and enzymes can be looked upon as molecules which mediate and run this show.
- Enzyme kinetics is the study of the chemical reactions that are catalyzed by enzymes, with a focus on their reaction rates.
- The study of an enzyme’s kinetics provides insights into the catalytic mechanism of the enzyme, its role in metabolism, how its activity is controlled in the cell, and how drugs and poisons can inhibit its activity.
- With the industries looking forward to simplify biochemical manipulations use of more and more enzymes have become inevitable replacements to the conventional chemical and microbial conversions.
The module includes
- Production & extraction of the enzyme
(You could choose from Amylase, Protease, Esterase, Invertase, Alkaline phosphotase, Phytase, Tannase, and many more from - plant, bacterial and fungal sources)
- Purification of the enzyme
- Determination of total activity, specific activity, folds purification, & percentage yield
- Determination of Km & Vmax
- Determination of optimum pH, Temp for the activity of the enzyme
- Study of the effect of changing enzyme, substrate, activator, inhibitor concentration on the activity of the enzyme
- Immobilization of the enzyme
- Determination of molecular weight of the enzyme
Fermentation Technology
- Fermentation, which is the heart of biotech industry, summarizes the end utility of all biotechnological research. It is the technology for production that will attend to most of the human needs, from enzymes to bio polymers, bio fuels, in the days to come.
- The use of fermenter is not only in production but also in standardization where optimizations of production processes are done to achieve maximum yield since the industrial profit hovers around the criteria of maximum output with minimum input.
- Immobilization is a break through technology in this arena which allows economical enzyme aided fermentation with reusability of used enzyme and reduced downstreaming.
Module:
- Screening of industrially important microorganisms
This involves isolation of the organism and screening for the production of the desirable product. - Biochemical characterization
- Media optimization
Standardization of production by varying carbon, nitrogen and mineral sources, pH and temperature - Shake flask Production
- Production in pilot scale
Analytical scale production in Scigenics stainless steel 3 litre fermenter - Downstream processing
Concentration, purification and characterization. - Immobilization
- Enzyme kinetics
Plant Biotechnology
- Plant biotechnology is a rapidly expanding discipline. It chiefly involves the introduction of foreign genes into economically important plant species, resulting in crop improvement and the production of novel plants.
- Plant biotechnology has many applications in the field of medicine, environment, food, farming and pharmaceutical industries.
Projects offered
- Studies on induction of organogenesis in various plant species
Explant culture, Direct regeneration, Indirect regeneration- Callus induction, regeneration of plant, Hardening. - Studies on induction of somatic embryos in various plant species
Explant culture, Direct regeneration, Indirect regeneration- Callus induction, suspension culture, viability test, Synthetic seed production, regeneration of plant, Hardening. - Studies on selection and screening of stress tolerant food crops
Standardization of sucrose concentration
Explant culture on Selective agent medium
Method to over come or to avoid the stress
Phenotypic trait estimation
Physiological estimation - Studies on somaclonal variation in various plant species
Explant culture for aseptic plantlets
Callus induction from different plant parts
Sub culturing of callus to increase the variation
Bio assay to know the susceptible concentration of selective agent
Culturing of callus on selective agent medium
Shoot induction
RAPD analysis - Production of plant secondary metabolites
Callus induction, Sub culturing of callus, Selection of cells, Suspension culture, Addition of elicitors to increase the metabolite production, Extraction, Concentration, Estimation through biochemical methods. - Agrobacterium mediated gene transformation in plants
- Explant culture for aseptic plantlets Culturing of Agrobacterium on broth disnfection of the explant
Co cultivation - Confirmation through PCR
- Identification of stress induced genetic variation in plants by RAPD analysis
- Standardization of sucrose concentration
- Explant culture on Selective agent medium
- RAPD analysis
MOLECULAR BIOLOGY
- Molecular Biology is the study of biological molecules and the molecular basis of structure and function in living organisms.
- Ever since the revolution in experimental biology occurred, the techniques in molecular biology and genetic engineering have been at the forefront and is still contributing immensely to the research being carried out globally.
Projects Offered
- Gene Cloning
- Isolation of the desired gene from a source
- Amplification of the gene by PCR
- Digestion of the gene of interest with suitable restriction enzymes.
- Ligation of the desired gene into a suitable vector.
- Transformation of the recombinant vector into the host cell
- Selection of desired clones
- Protein Expression, Purification and Detection.
- Transformation of host with the vector carrying the gene of interest
- Induction of Gene expression
- Purification of induced protein by Chromatography
- Detection of Induced Protein by Western Blotting
- DNA Fingerprinting
- RAPD
- Isolation of Genomic DNA from the different plant varieties
- Quantification of the isolated DNA
- PCR amplification with random primers
- Gel electrophoresis and analysis of the Gels in the Gel Documentation System.
- RFLP
- Identification of SNP’s
- Isolation of genomic DNA
- PCR amplification of region of interest
- Restriction Digestion with Specific Enzymes
- Analysis by Gel electrophoresis
Bioinformatics
Bioinformatics is the application of computer technology to the management of biological data. The data for analysis is obtained from techniques like DNA/Protein sequencing, X-Ray Crystallography, Gene expression studies etc. Analysis of such data includes identifying genes, detecting motifs of functional significance, predicting structure from sequence, studying evolutionary relationships etc. It is a rapidly developing branch of biology and is highly interdisciplinary, using techniques and concepts from informatics, statistics, mathematics, chemistry, biochemistry, physics, and linguistics. It has many practical applications in different areas of biology and medicine.
Modules
- Homology Modelling and Structure Analysis
- Sequence Analysis
- Phylogenetic Analysis
Source:
http://www.jivas.ac.in/
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July 18, 2009 |
Filed under Project Vacancies · Tagged with jivas bengaluru bioinformatics project, jivas enzymology project bengaluru, JIVAS project plant biotechnology
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